Mcf10a-ras

Author: cknm

August undefined, 2024

Web10 okt. 2024 · MCF10A-ras cells were transfected with ESRRA siRNA, and then 200 μM Eu was added to MCF10A-ras cells for 24 h. In some experiments, MCF10A-ras cells were … WebMCF10A- ras cells plated at a density of 2.5 × 10 4 cells/200 μL in 48-well plates were treated with SH48 and its analogues. After incubation for 24 h, cells were treated with the …

IJMS Free Full-Text Targeting WASF3 Signaling in Metastatic …

Web10 okt. 2024 · Treatment with Eu also significantly reduced intracellular ATP levels in MCF10A-ras cells but not in MCF10A cells. This effect was mediated mainly through … Web15 okt. 2015 · Motile/invasive MDA-MB-231 and Ras-MCF10A cells produce more endogenous hyaluronan, cell surface CD44 and Rhamm, … ian newbon barrister

Histone deacetylase inhibitor trichostatin A and autophagy …

WebMCF10A- Ras cells, and to a lesser extent MCF10A-c-Myc, showed reduced cell-cell contacts with a more polarized fibroblastoid appearance representing morphological changes associated with EMT... Web23 feb. 2015 · (a) Immunoprecipitation of GTP-bound KRAS from EV-transduced MCF10A cells or MCF10A clones expressing the indicated KRAS mutants at low levels following stimulation with EGF (20 ng/ml) for 10... WebMCF10a cells are a non-invasive, immortalized breast epithelial cell line and MCF10CA1a cells are a highly invasive, H-Ras transformed derivative of MCF10a cells selected for their metastatic potential. Using an in vitro Matrigel assay, MCF10CA1a cells showed much higher invasion than the parental MCF10a cells. mom wsh statistics

New model systems provide insights into Myc-induced …

MCF 10A - CRL-10317 ATCC

Web15th Feb, 2024. David Benz. Bucher Biotec AG. After 2 years of working with MCF10A cells I came to the conclusion that PEI works the best. The transfection efficiency is still low, but its better ... Web28 mrt. 2011 · The MCF10A-Myc cells, in contrast, were less responsive to this external cue and maintained a high level of deregulated Myc protein expression (Figure 1a, compare … ian newborn barristerWeb27 okt. 2024 · 1. Analysis of TGF-β-induced SMAD2 phosphorylation using Western blotting. NOTE: Premalignant human breast MCF10A-Ras cells were used as an example to investigate TGF-β signaling responses 33.In principle, the methods described below are also applicable to other TGF-β-responsive cell lines. ian newbold

"WebMCF 10A is an epithelial cell line that was isolated in 1984 from the mammary gland of a White, 36-year-old female with fibrocystic breasts. This cell line was deposited by the Michigan Cancer Foundation. Product category Human cells Organism Homo sapiens, human Cell type epithelial cell Morphology epithelial Tissue Breast; Mammary gland … " - Mcf10a-ras

Mcf10a-ras

Loss of giant obscurins from breast epithelium promotes epithelial …

Web26 mrt. 2009 · However, the mechanism by which SIM2s is repressed in breast cancer cells has not been determined. In this study, we show that transformation of MCF10A cells by … Web17 jan. 2024 · The proteins were extracted from MCF10A and MCF10A-ras cells treated with 0.5 µM TSA for 24 h. The expression of proteins was determined by western blotting analysis using the indicated antibody. β-actin was used as a loading control. TSA, trichostatin A; PI, propidium iodide; PARP1, poly-ADP-ribose polymerase-1.

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Web28 mrt. 2011 · The MCF10A cells were established through spontaneous immortalization of human mammary epithelium ( Soule et al., 1990 ). The non-transformed nature of this cell line is supported by the absence... WebActivation of the Ras pathway is also frequently found in breast ... into the mechanisms behind collective migration by studying cell migration in a …

Web3 jan. 2014 · Originally based on observations that human breast cancer cell lines contain mutationally activated ras oncogenes, and many breast carcinomas express higher levels of the ras proto-oncogene protein product than do normal tissues , , these cell lines were constructed to explore breast cancer progression and include lines representing … Webabilities of H-Ras MCF10A cells.To investigate the effect of ErbB2 on the invasion and migration of H-Ras-activated MCF10A cells, ErbB2 gene was introduced into H-Ras MCF10A cells by transfection, resulting a stable cell line ErbB2-H-Ras MCF10A. The intrinsic level of ErbB2 502 YONG et al: ErbB2/H-Ras ENHANCES …

Web22 jun. 2024 · Methods: The purpose of the current studies was to determine the effects of detachment on glucose and glutamine metabolism in human breast epithelial MCF10A … WebMCF 10A is an epithelial cell line that was isolated in 1984 from the mammary gland of a White, 36-year-old female with fibrocystic breasts. This cell line was deposited by the …

Web(C) MCF10A-ras cells were transfected with siH-RAS, then the cells were treated with 200 μM Eu. The protein expression of c-Myc, PGC-1β and ERRα were measured by western blot. β-actin was used ...

Web25 nov. 2024 · MCF10A-ras & MCF10A-ErbB2 [1,25(OH)2D] < 0.1 % in fresh medium changed every 24h 26 PLUCHINO 2015 Ergosterol MCF10A & CF12A 10nM 24h 30 WAHLER 2015 Vit D MCF10DCIS (basal-like BC) 1α25(OH)2D3: 100nM 24h 19 ZHENG 2013 Vit D (1,25(OH)2D ) MCF10A & MCF10A-ras 1,25(OH)2D 10nM for 4 days 29 … mom yearbook of statisticsWeb23 jan. 2024 · RAS is localized to the intracellular leaflet of the cell membrane where it serves as a major communication hub that relays extracellular growth factor-dependent … mom wsh postersWeb15 feb. 2010 · To gain insight into the role of genomic alterations in breast cancer progression, we conducted a comprehensive genetic characterization of a series of four cell lines derived from MCF10A. MCF10A is an immortalized mammary epithelial cell line (MEC); MCF10AT is a premalignant cell line generated from MCF10A by transformation … mom wsho applicationWeb7 okt. 2010 · MCF10A cells are a spontaneously immortalized human breast epithelial cell line, mutant in the cdk inhibitor p16, yet has many of the characteristics of normal breast epithelium, do not form tumors in nude mice nor form colonies in soft agar, but undergo transformation upon the introduction of Ha-Ras [ 33 – 35 ]. mom wsh policy templateWeb12 mrt. 2024 · We report the synthesis and preliminary characterization of IODVA1, a potent small molecule that is active in xenograft mouse models of Ras-driven lung and breast cancers. In an effort to inhibit oncogenic Ras signaling, we combined in silico screening with inhibition of proliferation and colony formation of Ras-driven cells. NSC124205 fulfilled … ian newbyWeb30 jun. 2024 · In the present study, we have investigated the effects of 15d-PGJ 2 on apoptosis of the Ha-ras transformed human breast epithelial (MCF10A-ras) cells. When … ian newbonWeb30 nov. 2010 · Both MCF10A cells and mouse fibroblasts (expressing empty vector) displayed a reduction in phosphorylated ERK1/2 levels following 24 h ECM detachment. In contrast, the phosphorylation of ERK1/2 remained elevated in both H-Ras V12 –transformed MCF10As and MEFs during ECM detachment ( Figure 2, A and B ). ian newby bracknell