WebJun 8, 2024 · They include eukaryotic translation initiation factor 2 subunit alpha (eIF2α), ras GTPase-activating protein-binding protein 1 (G3BP1), T-cell intracellular antigen-1 (TIA … WebFeb 4, 2024 · (T) Insulin/aa-stimulated G3BP1 KO cells generated with a second independent guide RNA against G3BP1 (sgRNA # 2, Table S1). Dashed line indicates cutting of immunoblot images to match the time points in (U) and (V). All time points were run on one gel. n = 3. (U) Quantitation of G3BP1 in (T). Shown are data points and mean …
G3BP1 protein expression summary - The Human Protein Atlas
WebApr 16, 2024 · GFP-tagged FKBP F36M-G3BP1 was transfected into U2OS G3BP1/2 dKO cells for 48 h, allowing the cells to reach 80% confluence. GFP-G3BP1 WT was used as … WebJan 25, 2024 · G3BP1 tethers the TSC complex to lysosomes and phenocopies lysosomal TSC functions. Like TSC1 and TBC1D7, G3BPs co-immunoprecipitated with TSC2 ( Figure 3 G). Thus, the TSC complex physically interacts with G3BPs. PLAs supported the association of G3BP1 with TSC2 in situ ( Figures 3 H, 3I, S4 A, and S4B). how to make a magnetic bookmark
UV damage induces G3BP1-dependent stress granule formation …
WebSurprisingly, G3BP1-depletion had no effect on cell death but caused the arrest of cell cycle in the G 0 /G 1 phase and increased the levels of p53 and p21. In addition, loss of G3BP1 led to a significant elevation of E-cadherin and decrease of N-cadherin, Vimentin, Snail, MMP-9, and MMP-2. Mechanistically, loss of G3BP1 dramatically suppressed ... WebFoot-and-Mouth Disease Virus Counteracts on Internal Ribosome Entry Site Suppression by G3BP1 and Inhibits G3BP1-Mediated Stress Granule Assembly via Post-Translational Mechanisms. Front Immunol. 2024 May 25;9:1142. ... Oxid Med Cell Longev. 2024 Jul 1;2024:3206542. (IF 5.076) 21. Luca M, Di Mauro M, Perry G WebMar 28, 2016 · We now show that cells lacking both G3BP1 and G3BP2 cannot form SGs in response to eukaryotic initiation factor 2α phosphorylation or eIF4A inhibition, but are still SG-competent when challenged with severe heat or osmotic stress. Rescue experiments using G3BP1 mutants show that phosphomimetic G3BP1-S149E fails to rescue SG … how to make a magnet with electricity