Cisplatin a549 xenograft

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August undefined, 2024

WebSep 14, 2024 · A549 cells were seeded in 96-well plates and cultured for 24 hours, then treated with varying concentrations of rhMG53 (0.05-100 μg/ml) or rhMG53 plus cisplatin (5 μM) for either 48 hours or 72 hours. Cells were detached by 0.25% Trypsin-EDTA solution, and their viability were quantified by flow cytometry (CytoFLEX S system, Beckman … WebFurthermore, combined treatment with intravenous injections of Lps-pVAX-LKB1 and intraperitoneal injection of low-dose cisplatin into mice bearing experimental A549 lung metastasis demonstrated significant (P<0.01) decrease in …

Cisplatin induces apoptosis of A549 cells by downregulating

WebJul 3, 2024 · (B) A549 xenograft tumors were treated with citrate (twice daily from day 14 to 40), cisplatin or citrate/cisplatin combination (3 doses of cisplatin at 4 mg/kg on days 14, 17 and 20 were given by ... WebThe Calu-6 cell line (human lung) is used to create the CDX (Cell Line Derived Xenograft) Calu-6 xenograft mouse model. The Calu-6 tumor model is an established xenograft model used to study the anti-tumor activity of therapeutics, such as bevacizumab, docetaxel, cisplatin and motesanib. how far is it from idaho falls to provo utah

Targeted therapy for cisplatin‐resistant lung cancer via …

WebDownload Table The 50 % inhibitory concentration (IC50) of cisplatin in A549 and A549/DDP cells 48 h after drug treatment from publication: RNA interferences targeting the Fanconi anemia/BRCA ... WebDownload scientific diagram The antitumour effect of CDDP and GA on A549 human xenograft models. (A) The mice transplanted with A549 human xenografts were … WebDec 1, 2024 · A549 cells, the human NSCLC cells with inherent cisplatin resistance, were used to investigate synergistic effect of sclareol with cisplatin in cell proliferation and migration as well as its regulatory mechanisms in expression of excision repair cross-complementation group 1 (ERCC1), a cisplatin resistance-associated molecule. how far is it from iceland to ireland

A549: Subcutaneous lung xenograft tumor mode - Reaction …

SRF231, a fully human CD47 antibody, potentiates the eﬀects …

WebMay 11, 2012 · We then evaluated the effects of LKB1 on enhancing low-dose cisplatin-mediated antitumor activities in both NSCLC cell lines in vitro and two human A549 lung … WebObjective: The purpose of the study was to investigate the role of peroxidase V (Prx V) in Cisplatin-induced apoptosis of A549 cells and its underlying mechanism. Materials and … how far is it from innisfil to fort erie onWebThe A549 cell line was derived from a human lung adenocarcinoma and has been used in numerous studies to investigate various aspects of lung cancer biology, including cell … high availability failover optimization

"WebMar 22, 2024 · In order to validate the underlying roles and mechanisms of TP53TG1 in enhancing the sensitivity of NSCLC cells to cisplatin, A549/DDP cells infected with lenti-control or lenti-TP53TG1 were subcutaneously injected into the nude mice to generate xenograft, followed by treatment with cisplatin or PBS. " - Cisplatin a549 xenograft

Cisplatin a549 xenograft

Diclofenac potentiates the antitumor effect of cisplatin in …

WebA standard immunohistochemistry (IHC) protocol was used to analyze tumor samples harvested from the A549R xenograft experiment. Briefly, the sections (5μm-thick) were first dewaxed and re-hydrated, and the endogenous peroxidase activity was blocked using 3% hydrogen peroxide. WebThe overexpression and knockdown of PUM2 promoted and inhibited tumor growth of xenograft mice, respectively. ... Cell viability of A549 and HCC827 cells treated with exosomes (25 μg/mL) derived from cisplatin-treated A549-R cells or CAFs. Control exosomes (Exo-control) derived from A549 or HCC827 cells’ own culture mediums. ...

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WebJun 3, 2024 · In A549 cells, cisplatin at 10 and 20 μM significantly induced early apoptosis, while TRIM65 overexpression significantly inducingcisplatin resistance in A549 cells (Fig. …

WebDec 1, 2024 · Cisplatin-based chemotherapy is a common first-line regimen for treating non-small cell lung cancer (NSCLC). However, drug resistance is still a major problem. … WebTargeting of the nano-zinc carrier to A549/DDP or A549 cells in vivo was detected after subcutaneous injection in a mouse xenograft tumor model. A549/DDP or A549 cells (1 …

WebFeb 1, 2008 · Subcutaneous A549 xenograft model. A549 human lung adenocarcinoma cells (5 × 10 6) resuspended in 100 μL PBS were injected into the dorsal flank of 6-week-old to 8-week-old male BALB/c nude mice. When tumor size reached ∼50 mm 3, mice were randomized into three groups ( n = 6 per group). WebFigure 6: SRF231 Administration Enhances Survival in the A549 Lung Orthotopic Xenograft Model A549-Luc tumor cells were implanted orthotopically into the lungs of SCID beige mice. Ten days postimplant, animals were sorted based on BLI imaging and treated IP with 800 μg SRF231 or human IgG4 isotype control antibodies biw x 3.

WebCisplatin: CDDP: A549, H358, H460 and Calu-1 cells: ... H460, H661, A549 cells and xenograft mouse models: Upregulating TF and TFR, downregulating SLC7A11 in a p53-dependent manner and increasing intracellular Fe 2+, …

Web2 days ago · Complex 11 showed a significant in vivo anticancer effect, revealed by its inhibiting effects on tumor growth in T24 xenograft mice without any renal pathological changes or influence on renal function. ... Complexes 36–40 were tested against lung carcinomas A549 and A549/DDP, cisplatin-insensitive cells, and normal healthy (HL … how far is it from indianapolis to st louisWebTargeting of the nano‐zinc carrier to A549/DDP or A549 cells in vivo was detected after subcutaneous injection in a mouse xenograft tumor model. A549/DDP or A549 cells (1 × 10 6 cells) in 50 μL of PBS were injected into the armpits of 4‐week‐old nude mice (BALB/c, nu/nu; SIPPR‐BK Laboratory Animal Co. Ltd, Shanghai, China) using a 25 ... high availability home assistantWebSep 2, 2024 · In the beginning, under both in vitro and in vivo conditions (A549 and H460 cells as well as A549 xenograft model) treated with external heat before and after intravascular administration of heat-activated thermosensitive liposome containing cisplatin (HTLC), exhibited rapid accumulation of drug in the tumor as well as a subsequent … how far is it from indiana to canadaWebJul 15, 2024 · The cisplatin-resistant A549 cell line (A549/DDP), the parent cell line A549, and 293 cells (JRDUN Biotech. Shanghai, China) were maintained in a humidified atmosphere with 5% CO 2 at 37 °C. high availability domain controllerWebOct 1, 2024 · A549 human lung carcinoma cells were obtained from the Korean Cell Line Bank (Seoul, Republic of Korea) and cultured in RPMI 1640 medium with 10% … how far is it from innisfil to erin onWebMay 18, 2024 · Our study showed that NO.0449-0145 treatment alone was able to overcome cisplatin resistance in A549-DDP cell and sensitize A549-DDP cells to cisplatin treatment, implying that the inhibition of ... high availability iis 10WebDec 1, 2024 · Xenograft Nephrotoxicity Antitumor effect 1. Introduction Cisplatin (CDDP) is a highly potent anticancer agent widely used for treating several types of cancers, … high availability firewall